Based on γ-ray emitting artificial radionuclide spectrometric measurements, an assessment of areal and vertical distribution of 137Cs, 60Co and 54Mn activity concentrations in bottom sediments of Lake Drūkšiai was performed. Samples of bottom sediments from seven monitoring stations within the cooling basin were collected in 1988–1996 and 2007–2010 (in July–August). For radionuclide areal distribution analysis, samples from the surface 0–5 cm layer were used. Multi sample cores sliced 2 cm, 3 cm or 5 cm thick were used to study the vertical distribution of radionuclides. The lowest 137Cs activity concentrations were obtained for two stations that were situated close to channels with radionuclide discharges, but with sediments that had a significantly smaller fraction of organic matter related to finest particles and consequently smaller radionuclide retention potential. The 137Cs activity concentration was distributed quite evenly in the bottom sediments from other investigated monitoring stations. The highest 137Cs activity concentrations in the bottom sediments of Lake Drūkšiai were measured in the period of 1988–1989; in 1990, the 137Cs activity concentrations slightly decreased and they varied insignificantly over the investigation period. The obtained 238Pu/239,240Pu activity ratio values in the bottom sediments of Lake Drūkšiai represented radioactive pollution with plutonium from nuclear weapon tests. Higher 60Co and 54Mn activity concentrations were observed in the monitoring stations that were close to the impact zones of the technical water outlet channel and industrial rain drainage system channel. 60Co and 54Mn activity concentrations in the bottom sediments of Lake Drūkšiai significantly decreased when operations at both INPP reactor units were stopped.