Orobanche crenata, Orobanche aegyptiaca and Orobanche foetida constitute serious threats to grain legumes in the Mediterranean Basin. Lentil can be severely infected by O. crenata. It can also be damaged although with less virulence by O. aegyptiaca, and can only be slightly infected by O. foetida. Resistance breeding is hampered by scarcity of proper sources of resistance in cultivated lentil and of a reliable and practical screening procedure. A germplasm collection of 23 wild Lens spp. accessions was screened for resistance to O. crenata under field conditions. A wide range of responses was observed, from complete resistance to susceptibility. The higher levels of resistance were observed in accessions of Lens ervoides, Lens odemensis and Lens orientalis. Resistance of selected accessions proved to be mainly because of early hampered tubercle formation, with no observed necrosis of established tubercles. No differences were observed in levels of induction of broomrape germination, but necrosis of broomrape radicles was significant in some cases, particularly in the L. odemensis–O. foetida interaction. Escape because of the reduced root biomass diminishing the chance of contact between host and parasite was also observed in L. odemensis.