Each map shown in Figure 4I–4S comparing the distribution of TC projections at the CSB between wild-type and Netrin-1−/− embryos have been divided into 12 bins along the rostrocaudal and dorsoventral axis of the CSB (A–H) or 25 bins in the DTh (I–L). Within each bin, two-way ANOVA test was used to determine the significance of the axon density maps between wild-type (WT) and Netrin-1 knockout embryos. Significance was arbitrarily set at p < 0.001, with green representing bins in which the averaged density observed in WT is superior to Netrin-1−/−, and vice versa for red bins. Any comparison with p > 0.001 was considered nonsignificant and shown in white. (1.61 MB TIF)