The removal ability of Fe(II) and Mn(II) by Eichhornia crassipes was investigated in this work. In an artificial batch condition, the removal efficiency of Fe(II) and Mn(II) reached 97% and 83%, respectively. The adsorption equilibrium of Mn(II) occurred before Fe(II) and more Fe(II) was taken up in mixed metal solution. The interrelationship between Fe(II) and Mn(II) revealed that both of them were essential elements for plants. Based on enrichment factor and translocation factor of E. crassipes, it was not only found that the metal ions were mainly adsorbed in the shoots but also further confirmed that E. crassipes had a higher affinity towards Fe(II). It could be concluded that E. Crassipes would be an ideal hydrophyte for removing Fe(II) and Mn(II) from aqueous solution.