Lignins were extracted from different species using organosolv process (ethanol/water). Obtained organosolv lignins were characterised by various methods to determine their composition, structure and functional groups with the purpose of evaluating their potential use for obtaining value-added compounds. The purity of organosolv lignins was determined. The total phenols content and the antioxidant power were analysed in order to know the reactivity of lignins. The ratio S/G was determined by nitrobenzene oxidation. In addition, molecular weight distribution, infrared spectroscopy and thermo gravimetrical analysis were carried out in order to determine the physical and chemical properties of organosolv lignins. Obtained organosolv lignins have very high purity and low sugar and inorganic contamination. All organosolv lignins samples have high polydispersity, and lignin from grey ironbark wood had the highest molecular weight average. Among the organosolv lignins, lemon-scented gum showed the highest average value of S/G ratio and the lowest average value of total phenols.