Canada has much potential to increase its already large use of hydroelectricity for energy production. However, hydroelectricity production in many cases requires the creation of reservoirs that inundate terrestrial ecosystems. While it has been reasonably well established that reservoirs emit GHGs, it has not been established what the net difference between the landscape scale exchange of GHGs would be before and after reservoir creation. Further, there is no indication of how that net difference may change over time from when the reservoir was first created to when it reaches a steady-state condition. A team of University and private sector researchers in partnership with Hydro-Québec has been studying net GHG emissions from the Eastmain 1 reservoir located in the boreal forest region of Québec, Canada. Net emissions are defined as those emitted following the creation of a reservoir minus those that would have been emitted or absorbed by the natural systems over a 100-year period in the absence of the reservoir. Sedimentation rates, emissions at the surface of the reservoir and natural water bodies, the degassing emissions downstream of the power house as well as the emissions/absorption of the natural ecosystems (forest, peatlands, lakes, streams and rivers) before and after the impoundment were measured using different techniques (Eddy covariance, floating chambers, automated systems, etc.). This project provides the first measurements of CO2 and CH4 between a new boreal reservoir and the atmosphere as the reservoir is being created, the development of the methodology to obtain these, and the first attempt at approaching the GHGs emissions from northern hydroelectric reservoirs as a land cover change issue. We will therefore provide: an estimate of the change in GHG source the atmosphere would see; an estimate of the net emissions that can be used for intercomparison of GHG contributions with other modes of power production; and a basis on which to develop biogeochemical sound, verifiable, and transparent estimates for GHG accounting. The results of the mass balance for this boreal reservoir from 2005 to 2009 as well as an extrapolation over 100 years will be presented.