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Wiley, Angewandte Chemie International Edition, 17(49), p. 3022-3025, 2010

DOI: 10.1002/anie.200906883

Wiley, Angewandte Chemie, 17(122), p. 3086-3089, 2010

DOI: 10.1002/ange.200906883

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A Facile Method To Encapsulate Proteins in Silica Nanoparticles: Encapsulated Green Fluorescent Protein as a Robust Fluorescence Probe

Journal article published in 2010 by Aoneng Cao, Zhangmei Ye, Zhengwei Cai, Erya Dong, Xiaowei Yang, Gangbo Liu, Xiaoyong Deng, Yanli Wang, Sheng-Tao Yang ORCID, Haifang Wang, Minghong Wu, Yuanfang Liu
This paper is available in a repository.
This paper is available in a repository.

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Abstract

(Figure Presented) Si'l vous plait? A facile and general method has been developed to encapsulate polyhistidine-tagged proteins in silica nanoparticles (NPs; gray, see picture) using calcium ions (yellow). The enhanced green fluorescence protein (EGFP) encapsulated in the silica NPs shows a substantial increase in fluorescence intensity and stability against denaturants, protease, and heat.