Background: Although it is relatively uncommon form of stroke, cerebral venous thrombosis (CVT) is still with difficulty in diagnosis and management. There is unsolved debate regarding the association of coagulation factor VII (FVII) R353Q polymorphism with venous thrombosis. Objective: We aimed to assess the association of FVII R353Q polymorphism with the risk of CVT. Methods: Patients with CVT and age and sex-matched controls were included in our study. All patients were subjected to history taking, examination, brain computerized tomography scans (CT), Magnetic resonance imaging (MRI) and magnetic resonance venography (MRV). For patients and controls; assay of FVII levels, and FVII R353Q polymorphism were assessed. Results: A total of 32 patients with CVT and 30 age and sex matched healthy subjects were enrolled in the study. There was no statistically significant difference between patients and controls regarding age, sex, body mass index, laboratory tests. Headache was the commonest presentation (91%) of our patients. Superior sagittal sinus thrombosis showed the highest prevalence. There was no statistically significant difference between patients and controls regarding the mean concentration of FVII (p= 0.43). Twenty two percent of our patients had the FVII 353RQ genotype compared to 13% of the control group while 78% of the patients had the 353RR genotype compared to 87% of the controls with no statistically significant difference between patients and controls. Conclusion: The study suggests no association between R353Q polymorphism for factor VII and both its plasma concentration as a risk of venous sinus thrombosis.