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Published in

The Company of Biologists, Biology Open, 4(2), p. 416-423, 2013

DOI: 10.1242/bio.20133780

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FAK–Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique

Journal article published in 2013 by Guangping Tai, Peter Hohenstein ORCID, Jamie A. Davies
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Summary This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK–Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.