This paper presents an optofluidic imaging system to detect influenza virus infection via co-culture of Madin Darby Canine Kidney (MDCK) cells. Influenza flu virus is a serious threat that can cause contagious infections in people in epidemic proportions. Hence, it is crucial to accurately detect and understand the morphological changes that occur in the cells when infected by the influenza virus. Recently, researchers are investigating the biophysical properties of cells and correlating them to biomedical conditions. For example, a decrease in refractive index (RI) is observed in bacterial infected cells [1]. In this paper, an optofluidic imaging system is developed to observe the change of RI in virus infected cells based on scattering signature.