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American Chemical Society, Journal of Proteome Research, 2(7), p. 786-794, 2008

DOI: 10.1021/pr700595p

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Resolving and Identifying Protein Components of Plant Mitochondrial Respiratory Complexes Using Three Dimensions of Gel Electrophoresis

Journal article published in 2008 by Etienne H. Meyer ORCID, Nicolas L. Taylor, A. Harvey Millar, A. Harvey Millar
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Analyzing highly hydrophobic proteins is a challenge for identification protocols based on gel separation and mass spectrometry. We combined Blue Native and 2D tricine gel electrophoresis to allow separation and identification of respiratory complex subunits from Arabidopsis mitochondria. We identified many of the highly hydrophobic mitochondrion-encoded subunits (GRAVY scores between +0.6 to +1.4) and also found a number of nucleus-encoded proteins associated with complex I for the first time in plants.