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Elsevier, Applied Surface Science, (313), p. 372-381, 2014

DOI: 10.1016/j.apsusc.2014.06.001

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Bioactive glass functionalized with alkaline phosphatase stimulates bone extracellular matrix deposition and calcification in vitro

Journal article published in 2014 by Enrica Vernè, Sara Ferraris, Chiara Vitale-Brovarone ORCID, Andrea Cochis, Lia Rimondini ORCID
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

Surface functionalization of bioactive glasses with the enzyme alkaline phosphatase (ALP) was studied in order to combine the inorganic properties of bioactive glass (bioactivity) with the biological properties of the biomolecule (mineralization). Two different bioactive glasses (SCNA and CEL2), with different degree of bioactivity, were used as substrates for the functionalization. ALP was grafted either by direct bonding or via an organic coupling agent. The cellular response to surface-modified bioactive glass was investigated using human osteosarcoma cells (MG63, osteoblast-like). Biocompatibility of all modified surfaces was verified. Of note, the more reactive glass (CEL2), modified with ALP, showed improved mineralization ability. © 2014 Elsevier B.V. All rights reserved.