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Elsevier, Bioorganic and Medicinal Chemistry Letters, 4(10), p. 365-368
DOI: 10.1016/s0960-894x(99)00700-3
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A novel thermophilic Glycosynthase that effects branching glycosylation
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Abstract
A novel thermophilic glycosynthase that effects branching glycosylation has been obtained by mutation of the nucleophile in the active site of the glycosidase from Sulfolobus solfataricus. Two methods for the use of this mutant are reported.