A total of 150 isolates of endophytic fungi were obtained from roots of Myrtus communis grown in the Mediterranean area. Seven different endophytic fungi taxa, all belonging to the Ascomycota phylum were found. Arbuscular mycorrhizal (AM) fungi, belonging to the Glomus genus, were also present in roots of M. communis. None of the root endophytic fungi tested, except Drechslera sp., colonized the root of sorghum. Among all fungi tested, only Phoma schachtii and Cylindrocarpon destructans significantly increased the shoot dry weight of sorghum grown in sterilized soil. Phomopsis columnaris, P. schachtii, Eucasphaeria sp. and Cylindrocarpon pauciseptatum increased the dry matter of sorghum grown in unsterilized soil. The percentage of AM colonization of sorghum was increased significantly only when Ph. columnaris, P. schachtii, the two strains of Bionectria ochroleuca, Eucasphaeria sp. and C. pauciseptatum were applied to unsterilized soil. The number of CFUs of endophytic fungi inoculated in sorghum rhizosphere was similar throughout the experiment, except B. ochroleuca-1 whose population increased at the end of the experiment either in sterilized or in unsterilized soil. No significant effect of the endophytic fungi isolated from M. communis on shoot and root P, K, Ca, Na, Mg, Cu and Zn concentrations of plants grown in sterilized or unsterilized soils, except Eucasphaeria sp. which increased the shoot P concentration of sorghum grown in unsterilized soil.