Purpose: To investigate the influence of 14 days live high, train low (LHTL) altitude exposure (14 h.d-1, at 3,000 m simulate altitude) on resting and post-exercise hepcidin and iron parameter responses in distance runners. Methods: Ten well-trained distance runners (six males, four females) performed a 6 × 1,000 m interval running session at 90% vVO2max in both normoxic (NORM) and simulated hypoxic (3,000 m) conditions (HYP) before, and again on the 11th and 14th day respectively of LHTL. Venous blood samples were collected after 48 h of altitude exposure, as well as before, and 3 h after all experimental trials. Blood samples were analysed for plasma hepcidin, iron, ferritin, transferrin and transferrin saturation (TSAT). Haemoglobin mass (Hbmass) was measured one week before, and two days after altitude exposure via CO rebreathing. Results: LHTL decreased resting hepcidin levels by 2.8 nM [95% CI; -3.7, -5.9] from 4.0 ± 1.2 nM to 1.2 ± 0.2 nM after 48 h (p < 0.01). NORM and HYP elevated hepcidin levels by 39.3% [8.0, 79.6] (p = 0.02) and 64.5% [16.9, 131.6] (p < 0.01) 3 h following interval exercise performed before LHTL. The post-exercise hepcidin response was not different following NORM (p = 0.10) and HYP (p = 0.37) performed during LHTL. Resting ferritin levels decreased by 27.6% [-37.9, -15.5] and 29.9% [36.2, 22.9] following 11 and 14 days of LHTL, respectively. Finally, LHTL increased Hbmass by 2.2% [1.0, 3.4] (p < 0.01). Conclusion: LHTL altitude exposure attenuated resting hepcidin levels but did not alter the magnitude of the post-exercise hepcidin response to normoxic and hypoxic interval exercise.