The differences in lipid composition between intracellular membranes cannot be adequately explained by local synthesis and degradation. Especially in the case of sphingolipids, which are synthesized in the Golgi complex but enriched on the cell surface and in endocytotic organelles, there is evidence for a cellular machinery that preferentially shuttles these lipids in vesicles to the cell surface. The machinery appears to involve the formation of domains of sphingolipid and cholesterol in the lumenal leaflet of Golgi membranes. Several pieces of evidence suggest that the selective anterograde transport of plasma membrane proteins may be mechanistically related to the sphingolipid domains.