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Published in

Oxford University Press, Nucleic Acids Symposium Series, 1(3), p. 297-298

DOI: 10.1093/nass/3.1.297

Wiley, Photochemistry and Photobiology, 2(74), p. 269-273

DOI: 10.1562/0031-8655(2001)0740269ioreds2.0.co2

Wiley, Photochemistry and Photobiology, 2(74), p. 269

DOI: 10.1562/0031-8655(2001)074<0269:ioreds>2.0.co;2

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Inhibition of Restriction Enzyme's DNA Sequence Recognition by PUVA Treatment†¶

Journal article published in 2007 by Fujinori Hanawa, G. H. Neil Towers, Mamoru Okamoto ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Applying various restriction enzymes on a specially designed 1.5 kb DNA fragment revealed that the inhibitory effects of PUVA treatment on restriction endonuclease activities are caused by recognition inhibition. In this study, Restriction enzymes which have a 5'-TpA sequence at the cleaving site (Kpn I, Xba I, Pme I, and Dra I), and non-cleaving site (Pac I) in recognition sites, or have two 5'-TpA sequences at the recognition site and a non-specific sequence between recognition and cleaving site (BciV I) were inhibited by PUVA treatment. Most of the other restriction enzymes used in this study which do not have a 5'-TpA sequence at their restriction site were not inhibited by PUVA treatment, although a 5'-TpA sequence is located adjacent (Sma I) or very close (BamH I, Sac I and Pst I) to the recognition and cleaving site for them. ; http://www.scopus.com/record/display.url?eid=2-s2.0-0142181130&view=basic&origin=inward&txGid=FbRdBUmXNO_mMN7PEPQMtws%3a8 ; Fujinori Hanawa, Mamoru Okamoto and G.H. Neil Towers