Published in

Wiley, Journal of Inherited Metabolic Disease, S2(12), p. 301-304

DOI: 10.1007/bf03335405

Studies in Inherited Metabolic Disease, p. 301-304

DOI: 10.1007/978-94-009-1069-0_34



Export citation

Search in Google Scholar

Prenatal Diagnosis of Zellweger Syndrome by Direct Visualization of Peroxisomes in Chorionic Villus Fibroblasts by Immunofluorescence Microscopy

This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Orange circle
Postprint: archiving restricted
Red circle
Published version: archiving forbidden
Data provided by SHERPA/RoMEO


Using a technique which makes use of digitonin to selectively permeabilize the cell, we established earlier that in chorionic villus fibroblasts catalase is contained in subcellular particles (peroxisomes) as in skin fibroblasts (Wanders et al., 1986). Based upon this finding was have now developed a sensitive method which allows direct visualization of peroxisomes in cultured chorionic villus fibroblasts by immunofluorescence microscopy using an anti-(catalase) antiserum, thus providing a simple method for prenatal diagnosis of Zellweger syndrome or any of the other peroxisome deficiency disorders.