Elsevier, Atherosclerosis, 1(162), p. 69-76, 2002
DOI: 10.1016/s0021-9150(01)00683-9
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The roles of transforming growth factor (TGF)-β1 in vascular proliferation, atherosclerosis, and plaque still remain controversial. TGF-β1 has been previously reported to inhibit the proliferation and migration of vascular smooth muscle cells and endothelial cells, in vitro. On the other hand, administration or transgenic overexpression of TGF-β1 enhances extracellular matrix synthesis and cellular hyperplasia of the intima and media in the normal artery and injured artery in vivo. We evaluated the correlation of arterial proliferation with plasma levels of TGF-β1 and TGF-β receptor type II, respectively, in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a new strain of spontaneous non-insulin-dependant diabetes mellitus (NIDDM) models. OLETF rats (n=30) were divided into three groups aged 5,15, and 30 weeks. Long-Evans Tokushima Otsuka (LETO) rats (n=30) were used as age-matched non-diabetic controls. Plasma TGF-β1 and insulin were determined by enzyme-linked immunosorbent assay. Immunoreactive TGF-β receptor type II antigen was detected by immunohistochemistry on the thoracic artery. Arterial media area was measured microscopically. Oral glucose tolerance test was performed to examine the stage of diabetes mellitus. The thoracic aorta wall section area increased significantly from the age of 15 weeks in OLETF rats, versus LETO rats. In both OLETF and LETO rats, plasma TGF-β1 increased significantly from the age of 15 weeks. In OLETF rats, plasma TGF-β1 increased significantly over that in LETO rats (P