Triatomines are insects that are taxonomically included in the Hemiptera order and Triatominae subfamily. Based on phenotypic similarity, capacity hybridization, and genetic and ecological aspects, the triatomine species can be grouped into specific complexes and subcomplexes. However, these groupings have not been confirmed. Cytogenetic analyses are important cytotaxonomic tools for improving the taxonomic knowledge of triatomines. Thus, we examined the karyotype of Triatoma baratai and compared the results with those of other species in the Matogrossensis subcomplex in order increase the understanding of vector potential. We also examined the cytotaxonomic classification of this insect. Triatoma baratai, similarly to other species that currently compose the Matogrossensis subcomplex, contains 22 chromosomes (20A + XY). Here, we describe the diploid chromosome set of Triatoma baratai. We confirmed their current classification in the Matogrossensis subcomplex and demonstrated that the species in this subcomplex present karyotype homogeneity. ; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)