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American Chemical Society, Journal of the American Chemical Society, 4(127), p. 1258-1264, 2005

DOI: 10.1021/ja045713m

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Confinement of Transmembrane Cell Receptors in Tunable Stripe Micropatterns

Journal article published in 2005 by Oliver Purrucker, Anton Förtig, Karin Lüdtke, Rainer Jordan ORCID, Motomu Tanaka
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We report a simple method to confine transmembrane cell receptors in stripe micropatterns of a lipid/lipopolymer monolayer, which are formed as result of the transfer onto a solid substrate. The stripes are aligned perpendicular to the meniscus, whose periodicity can systematically be tuned by the transfer velocity. This strongly suggests the dominant role of the cooperative interaction between the film and substrate. Selective fluorescence labeling of lipids and lipopolymers confirms that the observed patterns coincide with the demixing of two species. Covalent coupling of polymer headgroups enables us to use the stripe patterns as a support for a lipid bilayer membrane. Spreading of lipid vesicles with platelet integrin alphaIIbbeta3 on a self-assembled membrane micropattern demonstrates that cell adhesion receptors are selectively incorporated into the lipopolymer-rich region. The method established here provides us with a tunable template for the confinement of receptor proteins to geometrically control the cell adhesion.