Full genome sequencing provides numerous research opportunities, including the opportunity to study intra- and interspecific divergence of regulatory sequences within certain families. In eukaryotes, transcription begins with the binding of the TATA-binding protein (TBP) to the core gene promoter in the region of 30 bp upstream of the transcription initiation sites either specifically, in the presence of TATA box (TATA+), or nonspecifically, due to involvement of other proteins (TATA–). In this work, we found the effect of changes in the TATA+/TATA– status in the evolution of transcription factors ARF (Auxin Response Factors). The family of ARF genes, which mediate the primary response to auxin and regulate various morphogenetic processes, includes 23 genes in Arabidopsis thaliana and 25 genes in Oryza sativa. Having assessed the TBP/DNA affinity in promoters of genes of AtARF and OsARF families, we classified them as TATA+ (AtRF1, 3, 9–10, 12, 14–16, and 20–23; OsARF4, 5, 7, 10, 12, 16–18, and 25) and as TATA– (AtARF2, 4–8, 11, 13, and 17–19; OsARF1–3, 6, 8–9, 11, 13–15, 19, and 21–24). Our predictions on the TBP binding to the core promoter TATA+ of AtARF and OsARF genes correlated with the level of their expression in a series of experiments, indicating that this approach can be used to predict the TATA affinity to core promoters of plants. It was established that the TATA+/TATA– status of the AtRF gene is related to its protein function as a transcription repressor or activator (significance coefficient p < 0.025 according to Fischer’s exact test). Conversely, OsARF repressors of rice usually have the TATA– status (p < 0.005).