The hydrolysates from lipid-extracted microalgal biomass residues (LMBRs) were used as a source of nutrients for the cultivation of Chlorella vulgaris for lipid production under various CO(2) supply conditions, including different CO(2) concentrations and aeration rates. Both parameters had a significant effect on lipid production. A CO(2) concentration of 5% was found to be most suitable for microalgal growth. Microalga grew best at a CO(2) aeration rate of 0.5vvm. At this rate, biomass concentration and lipid productivity were at a maximum of 3.83gL(-1) and 157mg L(-1)d(-1), respectively, but decreased at lower or higher aeration rates. The present results showed that LMBRs utilization was effective in microalgal lipid production under suitable CO(2) supply conditions.