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Cold Spring Harbor Protocols, 11(2007), p. pdb.prot4608

DOI: 10.1101/pdb.prot4608

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Preparation and Use of Microcolumns for Sample Desalting or Nanoscale IMAC

Journal article published in 2007 by Hanno Steen, Allan Stensballe, Ole Nørregaard Jensen
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

INTRODUCTIONIn this method, microcolumns are prepared using any kind of chromatographic resin. The resin is held in place by making a constriction at the end of a GELoader pipette tip. Sample loading, washing, and elution are performed by loading liquid on top of the resin and applying air pressure to generate a low flow through the column. No frits are necessary.