This study aimed to perform an in vitro testing of the efficacy of various kinds of disinfectant on infectious bronchitis virus (IBV). Four kinds of disinfectants including Virusnip, Omnicide, CID2000 and Virkon S were used for the virus inactivation test. After challenge with each strain of IBV for each contact time (30 seconds, 1, 5 and 30 minutes), we recorded number of dead embryo/6 inoculated eggs after incubation. Results showed that there was no significant difference among the contact times except 1:800 Virusnip tested for Tha07 (p < 0.05). For Tha03, there was a significant difference among the disinfectants at 30 seconds and 1 minute of contact times (p < 0.05). For Tha08, there was a significant difference among the disinfectants at 1 minute of contact time (p < 0.05). For Tha09, there was a significant difference among the disinfectants at 5 and 30 minutes of contact times (p < 0.05). For Tha10, there was a significant difference among the disinfectants at 5 minutes of contact time (p < 0.05). Virusnip revealed the ability to inactivate the activity of 9 IBV strains in all exposure times, especially at dilutions of 1 : 100 and 1 : 200.