A Genetically Encoded Norbornene Amino Acid for the Mild and Selective Modification of Proteins in a Copper-Free Click Reaction
Journal article published in 2012 by
Emine Kaya,
Milan Vrabel,
Christian Deiml,
Stefan Prill,
Viviana S. Fluxa,
Thomas Carell 
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Abstract
Built in for clicks: A specially evolved pyrrolysyl-tRNA synthetase from Methanosarcina mazei was used to incorporate a norbornene amino acid into proteins in E. coli. This unit can be site-selectively modified under mild physiological conditions using a [3+2] cycloaddition reaction with nitrile imines.