The three-spined stickleback ( Gasterosteus aculeatus aculeatus L., 1758) shows unequal energetic expenditure upon reproduction as some males defend territories and build nest (territorials: TM) where they spawn singly with females, whereas other males remain nonterritorial (NTM), do not build nests, and resort to sneaking fertilizations from nesting males. We examined the effects of reproductive strategy on various sperm parameters. Territorial status was assessed by introducing males in small wading pools and recording the number of males with a nest. Males from all pools were remixed and nesting status was reassessed. This allowed us to discriminate a group of males with repetitive territorial or nonterritorial behaviours (TM and NTM) or a “facultative” territorial behaviour (FM). TM had a significantly larger gonadosomatic index (GSI) than both FM and NTM. FM had a GSI that was significantly larger than NTM, but significantly smaller than TM. Sperm motility did not differ among males with different reproductive tactics. There were no significant differences in citrate synthase (CS) and pyruvate kinase (PK) activities among males with different status. Curiously, we found a significant negative relationship between CS and PK activities and total number of sperm.