Dissemin is shutting down on January 1st, 2025

Published in

BioMed Central, Genetic Vaccines and Therapy, 1(9), 2011

DOI: 10.1186/1479-0556-9-5

Links

Tools

Export citation

Search in Google Scholar

B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis

Journal article published in 2011 by Luciana P. Almeida, Ana Pf F. Trombone, Julio Cc C. Lorenzi, Carolina D. Rocha, Thiago Malardo, Isabela C. Fontoura, Ana F. Gembre, Ricardo Ll L. Silva, Célio L. Silva, Ademilson P. Castelo ORCID, Arlete Am M. Coelho-Castelo
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Green circle
Published version: archiving allowed
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

Abstract Background Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. Methods In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. Results In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. Conclusions These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model.