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Microbiology Society, International Journal of Systematic and Evolutionary Microbiology, Pt_12(62), p. 2986-2990, 2012

DOI: 10.1099/ijs.0.037440-0

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Advenella faeciporci sp. nov., a nitrite-denitrifying bacterium isolated from nitrifying-denitrifying activated sludge collected from a laboratory-scale bioreactor treating piggery wastewater

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Strain M-07T was isolated from nitrifying–denitrifying activated sludge treating piggery wastewater. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain M-07T belonged to the genus Advenella . 16S rRNA gene sequence similarity between M-07T and Advenella incenata CCUG 45225T, Advenella mimigardefordensis DPN7T and Advenella kashmirensis WT001T was 96.5, 97.3 and 96.9 %, respectively. The DNA G+C content of strain M-07T was 49.5 mol%, which was approximately 5 mol% lower than the range for the genus Advenella (53.5–58.0 mol%). The predominant cellular fatty acids of strain M-07T were C16 : 0, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), C17 : 0 cyclo and summed feature 2 (comprising one or more of C14 : 0 3-OH, iso-C16 : 1 I, an unidentified fatty acid with an equivalent chain-length of 10.928 and C12 : 0 alde). The isoprenoid quinone was Q-8. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness, strain M-07T should be classified as a novel species of the genus Advenella , for which the name Advenella faeciporci sp. nov. is proposed. The type strain is M-07T ( = JCM 17746T = KCTC 23732T).