The diversity of phytoplasmas within single plants has not yet been fully investigated. In this project, deep amplicon sequencing was used to generate 50,926 phytoplasma sequences from 11 phytoplasma-infected grapevine samples from a PCR amplicon in the 5' end of the 16S region. After clustering and alignment to phytoplasma reference sequences it was shown that the phytoplas-mas in the 11 plants belonged to diverse 16Sr groups and that a high number of single nucleotide polymorphisms were present.