Anaerobic digestates (AD) from biogas production are applied to agricultural land as organic fertilizers, but pose an ammonia (NH3) emission source. However, data about NH3 emissions of cold season AD land spreading is still lacking. Therefore, in the present study NH3 emissions of AD application under winter conditions were determined. AD was applied via trail hoses to a field plot of 27 ha in Northern Germany during the winter with temperatures around the freezing point and partly frozen soil. NH4+ N application rate was, including a preceding urea application, 123 kg NH4+ and urea N ha−1. The NH3 volatilization was monitored using Open Path Fourier Transform Infrared spectroscopy in combination with a micrometeorological transport model. Cumulative NH3 volatilization during the six day measurements was 17.5 kg NH3 N ha−1 which corresponds to 33.1% of the NH4+ N in applied AD. This NH3 loss is relatively high for low temperature conditions and was most likely caused by the frozen soil restricting AD infiltration.