The effects of Chinese spices on the profiles of 17 heterocyclic amines (HAs) from seven HA categories were investigated in roast beef patties using UPLC-MS/MS and principal component analysis. Three groups of HAs, imidazopyridines (PhIP, DMIP and 1,5,6-TMIP), imidazoquinoxalines (MeIQx and 4,8-DiMeIQx) and β-carbolines (harman and norharman) were detected and quantified in all of the samples. The results demonstrated that the total HA and imidazopyridine profiles could clearly be affected by 1% pricklyash peel (14.1±0.76 and 6.06±0.32 ng/g), chilli (41.0±0.01 and 23.0±0.52 ng/g) and cumin (59.9± 2.44 and 31.1±3.06 ng/g), in comparison with control values of 21.8±2.40 and 14.3±2.04 ng/g, respectively. The difference was only significant (p<0.05) for cumin. The imidazoquinoxaline profile was significantly (p<0.05) affected by 1% pricklyash peel (0.57±0.05 ng/g) and cumin (2.36± 0.20 ng/g) compared to the control (1.16±0.11 ng/g). The β-carboline profile was only markedly (p<0.05) affected by 1% cumin (26.4±0.82 ng/g) compared to the control (6.26±0.26 ng/g). In general, pricklyash peel inhibited HA formation, whereas star anise, fennel, cumin, chilli and black pepper promoted HA formation. The findings could facilitate the selection of spices in meat processing to minimize HA formation.