American Society for Microbiology, Applied and Environmental Microbiology, 12(66), p. 5503-5505, 2000
DOI: 10.1128/aem.66.12.5503-5505.2000
Full text: Download
ABSTRACT Permeabilized cells of a highly enriched, toluene-mineralizing, methanogenic culture catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formation was >85% of the specific in vivo rate of toluene consumption. This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria.