Direct quantification of Asparagine (Asp) in a Streptomyces sp. M7 (SM7) culture medium was performed using multi-wall carbon nanotubes composite electrodes modified with copper microparticles (CNTPE-Cu). A sensitive and fast response is achieved at CNTPE-Cu after additions of SM7 cultures supernatant, without interference of the culture medium components. This response allows determining the Asp concentration as a function of the bacteria growth. The results reveal that the SM7 bacteria consume Asp as a carbon and nitrogen sources in the absence of another carbon source such as glucose.