The search for silvicultural information, including the seedling production methods, about alternative forest tree species for forestry with economic purposes have increased in the last years. In this work, the influence of the volume of tubes at sizes of 115, 180 and 280 cm3, on the growth of seedlings of Guanandi (Calophyllum brasiliense Cambess.) and Australian Cedar (Toona ciliata M. Roem. var. australis (F. Muell.)) was evaluated. The substrate used was composed by mixture of 80% of commercial substrate and 20% of clay. The collar diameter and the height of the seedlings were evaluated at 60, 90, 120 and 150 days after transplantation. In the last mensuration the dry weight of the aerial plant, of the root system and the total weight, and the Quality Index of Dickson were also determined. There was an effect of the tube volume on the growth and the quality of the seedlings, and the reaction of the Australian Cedar was greater. It was concluded the, for the Guanandi, the best tube is that of 180 cm3 and for the Australian Cedar is the tube of 280 cm3.