Asiatic clam (Corbicula fluminea) is one of the most invasive freshwater bivalves. Despite the ecologic and economic impacts of this species, there are only a few polymorphic microsatellites. In this study, we screened the genome from C. fluminea in search of microsatellite markers using massive parallel sequencing. We identified 246 new microsatellites involving di-, tri-, tetra- and pentanucleotide single repeats and different compound microsatellites, and we used a validation protocol to characterize a sample of those. Nine of them presented two or more alleles indicating a low level of variability.