The present study investigated the role of Testosterone on the regenerative capacity in two metamorphic stages of the tadpoles of the Egyptian toad, Bufo regularis Reuss, after amputation of the hind limb at the mid-shank level. It indicated an enhancing effect of Testosterone treatment on limb regeneration in the prometamorphic (stage 56), where 90% of the cases regenerated toes ranging from five to one compared with 77.3% in the control group, also the differential effect of testosterone on the number of toes was obvious in the treated animals, where 30% and 35% of the cases regenerated five and four toes respectively compared with 27.3% and 31.8% in the control group. In the metamorphic stage (stage58), the effect of testosterone was also obvious, where 38.6% of the treated cases restored toes compared with 13.3% of the cases in the control group. 45.5% of the treated cases restored part of the foot compared with 20% of the cases in the control group. Histological observations of the treated limbs revealed that the formation of thick epithelial covering and complete skin is faster than that of the control animals. This may indicate that the enhancing effect of testosterone on limb regeneration, this may be due to the acceleration of wound healing either by its action upon the proliferative phase of healing which involves immune processes such as reepithelialization and angiogenesis or by the production of IGF-1 or by its stimulatory effect through Wnt/β-catenin signaling resulting in the initiation of the early phases of limb regeneration.