A study on the seroprevalence of influenza virus in broilers was carried out. A total of 570 from broilers were randomly collected from 52 poultry farms of Quetta and its surroundings to determine the seroprevalence and antibody titre of avian influenza virus in the sera of broilers. The seroprevalence of avian influenza virus in the sera of was demonstrated by Enzyme Linked Immunosorbent Assay (ELISA). The sero-positive prevalence of avian influenza virus was recorded 14.03% (n=80) in broilers. All the positive sera of boilers determined by ELISA were further tested by using H 5 , H 7 , and H 9 specific strains antigen through Haemagglutination Inhibition and Haemagglutination tests, only H 9 was recognized from the sera of broilers. During present study the antibody titreof H9strain of avian influenza virus in the sera of broilers was investigated. The antibody titre of H 9 strain of avian influenza virus was measured in the sera of broilers ranged from 1:8 to 1:164. It is concluded from the present investigation that avian influenza virus H 9 strain is prevailing in broilers and around Quetta. _____________________________________________________________________________________________