Purpose: The purpose of this study was to test the reliability and validity of 2 standardized methods for calculating speed at the second lactate-threshold point (LT₂) based on the preexisting D_max (LT_D) and modified D_max (LT_MOD) procedures. Methods: 13 trained male road runners and triathletes completed 2 incremental laboratory running tests to determine LT₂, followed by separate time trials (5, 10, 15 km) on an outdoor running track. Two new methods were proposed for calculating the speed at LT₂: (1) the single standardized lactate threshold (LT_SDs) and (2) the paired standardized lactate threshold (LT_SDp) for quantifying changes over time. Results: The LT_SDs and LT_SDp methods had high relative (ICC ≥ .98) and absolute (CV ≤ 1.9%) reliability in identifying the speed at LT₂. The speed at LT2 according to the LT_SDs and LT_SDp methods had a strong correlation and was not different to the performance speed during the 10- and 15-km time trials (≤2.3%; ρ_c > 0.8; P > .05). The following natural logbased formula was created to estimate the percentage of LT₂ speed (using the LT_SDs method) that could be sustained for events ~15–75 min: y = –7.256(ln x) + 157.64, where y = %LT₂ speed, x = time-trial performance (s), and ln = natural log. Conclusions: The standardized methods are reliable for determining LT₂. The LT_SDs and LT_SDp methods for calculating the speed at LT₂ from a near-maximal incremental test calculated speeds similar to those exhibited in 10- and 15-km running time trials. A prediction equation for estimating the percentage of LT₂ that can be sustained for events of ~15–75 min was generated.