Published in

Optica, Optics Express, 26(15), p. 18275, 2007

DOI: 10.1364/oe.15.018275

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Characterizing and tracking single colloidal particles with video holographic microscopy

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.