Ensuring food security in the era of climate change is of major concern to the plant breeders worldwide. Wheat, being the staple food crop of the world, needs more focus by the scientifi c community for its genetic upgradation and devel-opment of cultivars tolerant to prevalent biotic and abiotic stresses. The role of alien gene introgression in utilization of wild genetic resources to enhance the genetic diversity in the cultivated wheat varieties is widely acknowledged. Classical approaches of alien gene transfer like wide hybridization have been practiced to a great extent but are hindered by a number of factors like linkage drag and poor cross-ability among the species. When such approaches are coupled with novel biotechno-logical tools, they allow swift, precise and targeted gene transfer. Transgenic approach offers a great advantage in alien gene transfer by keeping aside the problems encoun-tered in previous approaches and opens new avenues for alien gene transfer in wheat, hence ensuring broadening and diversifi cation of wheat genome. This chapter focuses on the successful attempts of alien gene introgression into wheat through various approaches along with their limitations and future prospects.