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Elsevier, Biomolecular Detection and Quantification, (2), p. 1-3, 2014

DOI: 10.1016/j.bdq.2014.11.003

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Making standards for quantitative real-time pneumococcal PCR

Journal article published in 2014 by Susan C. Morpeth ORCID, Jim F. Huggett, David R. Murdoch, J. Anthony G. Scott ORCID, J. Anthony G. Scott
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.