Late Holocene glacier variations in westernmost Tibetan Plateau were studied based on the analysis of grainsize, magnetic susceptibility, and elements from an 8.3-m long distal glaciolacustrine sediment core of Kalakuli Lake. Our results show that there are four glacier expansion episodes occurring in 4200–3700 cal yr BP, 2950–2300 cal yr BP, 1700–1070 cal yr BP, and 570–100 cal yr BP, and four glacier retreat periods of 3700–2950 cal yr BP, 2300–1700 cal yr BP, 1070–570 cal yr BP, and 50 cal yr BP-present. The four glacier expansion episodes are generally in agreement with the glacier activities indicted by the moraines at Muztag Ata and Kongur Shan, as well as with the Late Holocene ice-rafting events in the North Atlantic. Over the last 2000 years, our reconstructed glacier variations are in temporal agreement with reconstructed temperature from China and the Northern Hemisphere, indicating that glacier variations at centennial time scales are very sensitive to temperature in western Tibetan Plateau.