An optimization study of biopolymer production by locally isolated Bacillus sp. strain-R was carried out, where analysis indicated that it is a polyamide homopolymer consists mainly of glutamate. Preliminary experiment to address the most suitable carbon and nitrogen sources revealed that a production of 14.25 g L^-1 of the polyamide (polyglutamate) was achieved in the presence of glucose and ammonium sulfate. To evaluate the effect of different culture conditions on the production of PGA, Plackett-Burman factorial design was carried out. Ten variables were examined for their significance on PGA production. Among those variables K₂HPO₄, KH₂PO₄, (NH₄)₂SO₄ and glucose were found to be the most significant variables that encourage PGA production. The pre-optimized medium showed approximately three folds increase in PGA production.