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National Academy of Sciences, Proceedings of the National Academy of Sciences, 3(98), p. 1042-1046, 2001

DOI: 10.1073/pnas.98.3.1042

National Academy of Sciences, Proceedings of the National Academy of Sciences, 3(98), p. 1042-1046

DOI: 10.1073/pnas.031562998

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Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands

Journal article published in 2001 by Hui Miao, Miguel Angel del Pozo ORCID, Yi-Shuan Li, Shila Jalali, Kuang-Den Chen, Martin A. Schwartz, John Y.-J. Shyy, Shu Chien
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The aim of this study is to elucidate the role of integrins in transducing fluid shear stress into intracellular signals in vascular endothelial cells, a fundamental process in vascular biology. We demonstrated that shear stress activates specific integrins in endothelial cells plated on substrates containing the cognate extracellular matrix (ECM) ligands. The shear stress-induced mechanotransduction, as manifested by integrin–Shc association, was abolished when new integrin–ECM ligand interactions were prevented by either blocking the integrin-binding sites of ECM ligands or conjugating the integrins to immobilized antibodies. Our results indicate that the dynamic formation of new connections between integrins and their specific ECM ligands is critical in relaying the signals induced by shear stress to intracellular pathways.