Taylor and Francis Group, OncoImmunology, 1(5), p. e1052213, 2015
DOI: 10.1080/2162402x.2015.1052213
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In our previous studies we have shown that patients with serous ovarian carcinoma in advanced surgical stage disease have a particularly poor prognosis if they carry the HLA-A*02 genotype. This represent a stronger prognostic factor than loss or down regulation of the MHC-class I heavy chain (HC) on tumour cells. In this study, we investigated the expression of the non-classical, immune tolerogenic HLA -G and –E on the tumour cells along with the infiltration of immune cells in the tumour microenvironment. FFPE primary tumours from 72 patients with advanced stages of serous adenocarcinoma and metastatic cells present in ascites fluid from 8 additional patients were included in this study. Both expression of HLA-G and aberrant expression of HLA-E were correlated to a significant worse prognosis in patients with HLA-A*02, but not with different HLA genotypes. Focal cell expression of HLA-G correlated to a site-specific down regulation of classical MHC class I HC products and aberrant HLA-E expression, showing a poor survival. HLA-G was more frequently expressed in metastatic cells than in primary tumour lesions and the expression of HLA-G inversely correlated with the frequency of tumour infiltrating immune cells. All these parameters can contribute together to identify and discriminate subpopulations of patients with extremely poor prognosis and can give them the opportunity to receive, and benefit of individually tailored treatments.