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Penetration problems are important in many areas of geotechnical engineering, such as the prediction of pile capacity and interpretation of in situ test data. The cone penetration test is a proven method for evaluating soil properties, yet relatively little research has been conducted to understand the effect of soil layering on penetrometer readings. This paper focuses on the penetration of a probe within layered soils and investigates the layered soil effects on both penetration resistance and soil deformation. A series of centrifuge tests was performed in layered configurations of silica sand with varying relative density in a 180° axisymmetric model container. The tests allowed for the use of a half-probe for observation of the induced soil deformation through a poly(methyl methacrylate) window as well as a full-probe for measurement of penetration resistance within the central area of the container. The variations of penetration resistance and soil deformation characteristics as they relate to penetration depth, soil density and soil layering are examined. The results of deformation are also compared with previous experimental data to examine the effect of the axisymmetric condition. The effects of soil layering on both resistance and soil deformation are shown to be dependent on the relative properties between soil layers.