Published in
Elsevier, Journal of Microbiological Methods, (106), p. 55-56
DOI: 10.1016/j.mimet.2014.07.027
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Detection of the Helicobacter pylori dupA gene is strongly affected by the PCR design
Journal article published in 2014 by
Amin Talebi Bezmin Abadi 
,
Ruud J. L. F. Loffeld,
Ashandra C. Constancia,
Jaap A. Wagenaar,
Johannes G. Kusters
,
Ruud J. L. F. Loffeld,
Ashandra C. Constancia,
Jaap A. Wagenaar,
Johannes G. Kusters
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Abstract
The Helicobacter pylori virulence gene dupA is usually detected by PCR, but the primer binding sites used are highly variable. Our newly designed qPCR against a conserved region of dupA was positive in 64.2% of 394 clinical isolates while the positivity rate of the commonly used PCRs ranged from 29.9% to 37.8%.