Catalyst utilisation properties of an 85 wt% Pt-Ru (1:1 a/o)/C anode and a 60 wt% Pt/C cathode in Direct Methanol Fuel Cells (DMFCs) operating at low temperatures (30 • C-60 • C) was investigated. The catalysts were in-house prepared and characterised in terms of structure and morphology. The influence of noble metal loading on the performance of low temperature DMFCs was investigated by steady-state polarisation measurements. A stripping voltammetry analysis of the adsorbed methanolic residues was carried out in-situ to get information on electrode activity and catalyst utilisation. The DMFC maximum power density increased linearly from 30 to 75 mW·cm −2 at 60 • C passing from 1 to 5 mg·cm −2 Pt loading in both electrodes. By further increasing the Pt loading at 10 mg·cm −2 only a slight increase of power density was recorded (81 mW·cm −2). Moreover, a decrease of catalyst utilisation was observed in the latter case. Furthermore, a decrease of Pt anode loading to 2 mg·cm −2 whilst maintaining the cathode loading at 5 mg·cm −2 did not result in significant performance degradation. The present analysis indicates that, in the low temperature range, a Pt loading of 5 mg·cm −2 at the cathode and 2 mg·cm −2 at the anode could be a suitable compromise in terms of performance, tolerance to cross-over effects and optimisation of noble metal loading allowing DMFCs to cover the power requirements of a wide number of portable devices.