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Albumin Genetic Diversity Analysis of Faba Bean (Vicia faba L.) Germplasms

Journal article published in 2012 by Jian Bin Dan, Mo Wei Hou, Yu Jiao Liu, Xiao Gang Ma
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

In order to investigate genetic diversity of albuminous loci, 101 genotypes of faba bean (Vicia faba L.) were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE). A total of 2625 protein bands were detected in tested genotypes. Except common bands, the protein bands of 92KDa, 75KDa, 62KDa, 40KDa, 34KDa, 17KDa and 13KDa presented the highest frequencies of 92.08%, 90.10%, 99.01%, 95.05%, 95.05%, 98.02% and 95.05% respectively. The other 29 polymorphic protein bands showed higher polymorphism with 16.09 polymorphic bands in average. Each genotype had 2131 albumin bands, with an average of 25.99. The genetic similarity (GS) of the tested genotypes varied from 0.6111 to 0.9722, with an average of 0.7122. The genetic diversity index within group (Hgroup=0.9879) was much higher than that among groups (Hbetween=0.0121) , which indicated the main variation of albumin occurred within populations. Cluster analysis divided the accessions into six major clusters, which somehow coincided with systematic classification of faba bean in previous studies of genetic resources. The research results provided reference to faba bean breeding on protein qualities.